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We collected wild mushroom, Sarcodon aspratus from Ducku Mountain in Jeollabuk-do of Korea and physiological functionalities of its water extract were investigated.The water extracts from Sarcodon aspratus showed the highest antihypertensive angiotensin I-converting enzyme (ACE) inhibitory activity of 74.3% and also showed high anti-gout xanthine oxidase (XOD) inhibitory activity (59.6%).However, tyrosinase inhibitory activity was very low (17.3%), and antioxidant activity and SOD-like activity were not detected.The ACE inhibitor from the fruiting body of S.aspratus was maximally extracted when powder of the fruiting body was shaked at 30℃ for 24h by distilled water.Furthermore, the XOD inhibitor was also maximally extracted by treatment at 50℃ for 24h with distilled water.