【摘 要】
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Objective To investigate the different effects of salvianolic acid and Notoginseng triterpenes on proliferation, angiogenesis and expression of vascular endothelial growth factor in EA-hy926 cells in
【机 构】
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Jiangsu province hospital of traditional Chinese medicine,Nanjing,P.R.China
【出 处】
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2013中国医师协会中西医结合医师大会
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Objective To investigate the different effects of salvianolic acid and Notoginseng triterpenes on proliferation, angiogenesis and expression of vascular endothelial growth factor in EA-hy926 cells in vitro.Methodls EA-hy926 cells were cultured in vitro.Salvianolic acid and Notoginseng triterpenes at concentrations of 0.4, 0.8 and 1.2 mg/L were used to culture EA-hy926 cells.And EA-hy926 cells in blank control group were cultured with culture solution only.Viability of cells was assessed by CCK-8, and after treated for 12h, capillary-like structures were examined.After 24-hour culture, the expression of VEGF was detected by real-time PCR.Fesults Salvianolic acid at 0.4, 0.8 mg/L, the same as Notoginseng triterpenes, increased VEGF content in EA-hy926 cells.Expression of VEGF protein in salvianolic acid at 1.2mg/L group, was up-regulated as compared with Notoginseng triterpenes group (P<0.05).Conclusion salvianolic acid and Notoginseng triterpenes can promote EA-hy926 cells proliferation, angiogenesis and expression of VEGF protein.Our analysis also provided evidence that salvianolic acid had the better effects as compared with Notoginseng triterpenes.
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