高效快速甘蔗转基因方法探索

来源 :中国作物学会甘蔗专业委员会第15次学术研讨会 | 被引量 : 0次 | 上传用户：ghz2000

【摘要】

：

甘蔗遗传背景复杂,转基因技术是甘蔗品种遗传改良的重要途径.甘蔗转基因遗传改良主要是通过基因枪法和农杆菌介导法来实现,但是目前我国的甘蔗转基因技术,普遍存在着转基因效率偏低,获得的转基因株系偏少,难以筛选到目的基因稳定表达,有利用价值的转基因株系等问题.本实验室通过多年的经验积累,建立了一种高效的快速的农杆菌介导法的甘蔗转基因方法.转化效率为20％左右,筛选效率达到了90％,转化时间为4个月左右,操

【作者】

：

王文治杨志坚熊国如蔡文伟冯翠莲王俊刚伍苏然张树珍

【机构】

：

中国热带农业科学院热带生物技术研究所甘蔗研究中心,海南海口,571101 福建农林大学农产品品质研

【出处】

：

中国作物学会甘蔗专业委员会第15次学术研讨会

【发表日期】

：

2014年6期

【关键词】

：

甘蔗农杆菌介导法转基因技术转化效率

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

其他文献

汇聚正能量,全力推进蔗糖产业提质增效

本文阐述了德宏州蔗糖产业发展取得的成效,全面分析了当前蔗糖生产面临的主要问题,提出全力推进蔗糖产业提质增效需要统一思想，加强领导，切实加强蔗糖产业建设，规划良好的蔗区布局，制定和落实良好的扶持政策，积极争取上级支持，整合项目资源，改善蔗区基础设施条件等措施。

会议

蔗糖产业行业分析战略管理布局规划

Comprehensive Selection of Reference Genes for Gene Expression Normalization in Sugarcane by Real Ti

The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s).In orde

会议

Molecular characterization and phylogenetic analysis of Sugarcane yellow leaf virus isolates from Ch

Sugarcane yellow leaf virus (SCYLV) (genus Polerovirus,family Luteoviridae),the causal agent of sug arcane yellow leaf disease (YLD),was first detectedin China in 2006.To assess the distribution of SC

会议

Genomic sequencesPhylogenetic relationshipsSugarcane (Saccharum spp. hybrid)S

Establishment and application of a loop-mediated isothermal amplification (LAMP) system for detectio

会议

MOLECULARENGINEERING IN PLANTS AGRICULTURAL GENETICSCOMPARATIVE GENOMICSPLANT

Genome characterization of sugarcane yellow leaf virus from China reveals a novel recombinant genoty

Sugarcane yellow leaf virus (SCYLV; genus Polerovirus,family Luteoviridae) is a recombinant virus associated with yellow leaf disease,a serious threat to sugarcane in China and worldwide.Among the nin

会议

Enhanced Transgene Expression in Sugarcane by Co-Expression of Virus-Encoded RNA Silencing Suppresso

Post-transcriptional gene silencing is commonly observed in polyploid species and often poses a major limitation to plant improvement via biotechnology.Five plant viral suppressors of RNA silencing we

会议

A novel dirigent protein gene with highly stem-specific expression from sugarcane, response to droug

Dirigent and dirigent-like family proteins contain a number of proteins involved in lignification or in the response to pathogen infection and abiotic stress in plants.In the present study,a full-leng

会议

Sugarcane(Saccharum spp.)Dirigent proteinProkaryotic expressionReal-time qPCR

Cultivar Evaluation and Essential Test Locations Identification for Sugarcane Breeding in China

会议

Isolation of a Novel Peroxisomal Catalase Gene from Sugarcane, Which Is Responsive to Biotic and Abi

Catalase is an iron porphyrin enzyme,which serves as an efficient scavenger of reactive oxygen species (ROS) to avoid oxidative damage.In sugarcane,the enzymatic activity of catalase in a variety (Yac

会议

Genetic Analysis of Diversity within a Chinese Local Sugarcane Germplasm Based on Start Codon Target

会议

高效快速甘蔗转基因方法探索

与本文相关的学术论文