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In this study,we aimed to investigate the clinical and prognostic value of a 5-gene expression signature model for gastric cancer patients upon microarray data.A total of 158 gastric cancer patients were selected,with 33 cases used for microarray analysis as training set and 125 cases for validation real-time quantitative polymerase chain reaction analysis as test set.Unsupervised clustering algorithms and supervised clustering algorithm were used to identify differentially expressed genes.