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Encephalomyocarditis virus(EMCV)can cause acute myocarditis in young pigs or reproductive failure in sows.In this study,an EMCV strain(BD2)was isolated from the suspected piglets with EMCV in China.It was identified by an indirect immunofluorescence assay and reverse-transcription polymerase chain reaction.The virus could reproduce on BHK-21 cells and reach a peak titer by 16hpi with maximum titers of 108.22 TCID50/0.1ml.