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Purpose: JC virus（JCV） infects 80-90% of the population, and results in progressive multifocal leukoencephalopathy upon immunodeficiency. The study aimed to pathologically clarify the oncogenic roles of T antigen in human breast cancers.Methods: Breast cancers, dysplasia and normal tissues were examined for T antigen of JCV by nested and real-time PCR. The positive rate or copy of T antigen was compared with clinicopathological parameters of breast cancer. JCV existence was morphologically detected by immunohistochemistry and in situ PCR. T antigen was examined by Western blot using frozen samples of breast cancer and paired normal tissues.Results: According to nested PCR, the positive rate of breast ductal or lobular carcinoma was lower than normal tissue（P＜0.05）. T antigen existence was negatively correlated with E-cadherin expression and triple-negative breast cancer（P＜0.05）, but positively with lymph node metastasis, estrogen receptor and progestogen receptor expression（P＜0.05）. Quantitative PCR showed that JCV copies were gradually decreased from normal tissue, dysplasia to cancer（P＜0.05）. JCV T antigen copy was lower in ductal adenocarcinoma than normal tissue（P＜0.05）, in line with situ PCR and immunohistochemistry. JCV copies were negatively correlated with tumor size and E-cadherin expression（P＜0.05）, but positively with G grading of breast cancer（P＜0.05）. Western blot also indicated weaker T antigen expression in breast cancer than normal tissues（P＜0.05）.Conclusions: JCV T antigen might play an important role in breast carcinogenesis. It can be employed as a molecular marker for the differentiation and aggressive behaviors of breast cancer.