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An investigation was carried out to detect the seropositivity of anti-Toxoplasma gondii antibodies in 11 Royal Bengal tigers,3 Asiatic lions,2 leopards and 4 elephants in winter and monsoon seasons by indirect ELISA.The total numbers of positive and suspected cases in winter season were 5 %(1/20) and 20 %(4/20),respectively.In monsoon season,the number of the infected individuals (4/20) exceeded the number of suspected ones (1/20).Leopards and elephants demonstrated negative titres in both the seasons.