Cordyceps militaris, an edible and medicinal fungus, in this study the process of extraction from C.militatis by using ultrahigh pressure (UHP) treatment combined with cellulase was investigated.The optimum extraction condition is proved to be the UHP treated material(400MPa, 8min) then cellulase hydrolysis (enzyme loading of 1.0uL/mL, 50℃, pH4.5and 4h) and distilled water extraction(55 ℃ , 2h, solid to liquid ratio of 1∶10, extract twice).UHP treatment followed by cellulase hydrolysis provided a high increase in the performance of extractability of the active substances from C.militaris.The yield of crude extract from C.militaris increased from 50% to70%, compared with only UHP treatment or cellulase hydrolysis.The active substance content of the protein, cordycepin and polysaccharide in the crude extract can be greatly enhanced from 11.24% to 22.07%, 0.214% to 0.616% and 22.50% to 28.25%, respectively.Based on SEM observation, it showed that UHP can result in disruption of microbial cells and destruction of recalcitrant cell wall polymers.It can help release the hidden macromolecules and improved the accessibility of cellulose.So the UHP treatment followed by cellulase hydrolysis can increase the extractability.