Seed oil content can greatly affect the yield of vegetable oil in rapeseed(Brassica napus L.).Therefore,increasing seed oil content has always been the goal of rapeseed breeding.Rapeseed oil content is a quantitative trait and controlled by multiple genes.In this study,QTLs on A08 are confirmed again and 10 new significant associations of oil content were obtained by GWAS and map-based QTL mapping methods.