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Tox21 has got into the third phase and clearly put forward the research direction of the next 5-10 years, including using cells derived from human and lower organisms as the experimental model, and combined with the technology platforms of high content, high throughput and transcriptomics to evaluate the potential toxicity of chemicals.Toxicity assays in the model organism nematode Caenorhabditis elegans (C.elegans) can be fast and inexpensive; however few studies have been performed comparing toxic responses in the nematode with data on rodent toxicity.First of all, we assayed the acute toxicity of 21 chemicals using C.elegans.The nematodes were exposed to different concentrations of chemicals in 96-well plate for 24 h.The median lethal concentration (LC50) and median lethal times (LT50) were calculated.The results indicated that excluding 4 types of acidic chemicals, there were positive correlations between LC50s of C.elegans and LD50s of mouse/rat (r>0.72, p<0.01) after both 12-h and 24-h exposure.Both of LT50 and LC50 of C elegans can be utilized as toxic effect index for further study on acute toxicity test of chemicals.Furthermore, we have exploratively built a more efficient chemical toxicity screening technology system based on the microfludic chip of high throughput and C.elegans, which will construct a new in vivo model of fast pre-screening the new chemicals or complex chemicals, as well as provide basic data to further decide the priority of the chemical toxicity test in rodent.By steps of chip template design, template production, chip pouring and platform establishment, we built up a microfludic chip platform, which could provide programmatically food and medium, wash the larvae, acquire image data automatically, and observe 16 phenotypic indices of toxicity to C.elegans.Sub-chronic toxicity test of 6 GHS classic chemicals (i.e.MnCl2), to C.elegans based on the microfludic chip system indicates that development and motility related indices are more sensitive and target toxicity of chemicals is quite significant in the detection of chemical toxicity.In summary, C.elegans may be a valuable model for predicting chemicals potential toxicity in rodent.