Ovarian carcinoma is the most lethal gynecologic malignancy;the majority of patients die within 5 years of diagnosis.The poor survival rate is attributed to diagnosis at advanced stage, when the tumor has metastasized.The epithelial-to-mesenchymal transition (EMT) is a necessary step toward metastatic tumor progression.Through integrated computational analysis, we recently identified a master microRNA (miRNA) network that includes miR-101 and regulates EMT in Ovarian carcinoma.In this study, we characterized the functions of miR-101.Using reporter gene assays, we demonstrated that miR-101 suppressed the expression of E-cadherin repressors ZEB1 and ZEB2 by directly targeting the 3-untranslated region of both ZEB1 and ZEB2.Introduction of miR-101 significantly inhibited EMT and cell migration and invasion.Introducing cDNA of ZEB1 and ZEB2 without 3UTR abrogated miR-101-induced EMT alterationrespectively.Our findings revealed that miR-101 represents a redundant mechanism for the miR-200 family that regulates EMT through two major E-cadherin transcriptional repressors.