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In vitro and in animal studies have suggested an important role for the Mycobacterium tuberculosis PE_PGRS33 protein in the pathogenesis ofTB.A significant level of PE_PGRS33 gene DNA polymorphism among clinical isolates from adult tuberculosis (TB) patients and its association with clinical and epidemiological phenotypes of the disease have been found.To better understand the role of PE_PGRS33 protein in the pathogenesis pediatric TB, we' investigated DNA polymorphism of the PE_PGRS33 gene among 101 pediatric TB patients' isolates and assessed the relationship between the PE_PGRS33 sequence variation and clinical characteristics of TB.Twelve different PE_PGRS33 sequence variations representing 12 different alleles were observed among the 101 M.tuberculosis clinical isolates investigated.Of these 101 isolates, 62(59.41%) had PE_PGRS33 alleles that would result in a change in the amino acid sequence of the PE_PGRS33 protein.The degree of DNA polymorphism within individual M.