【摘 要】
：
Objective To develop a colloidal gold immunochromatographic assay for rapid detection of NJ001 specific antigen.Methods Polyclonal antibody was prepared by immunizingNew Zealandrabbit with SPC-Al cell
【机 构】
：
Department of Laboratory Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjin
【出 处】
：
中华医学会第八次全国中青年检验医学学术会议
论文部分内容阅读
Objective To develop a colloidal gold immunochromatographic assay for rapid detection of NJ001 specific antigen.Methods Polyclonal antibody was prepared by immunizingNew Zealandrabbit with SPC-Al cells.Murine monoclonal antibody NJ001 was conjugated with colloidal gold particles prepared by the citrate method,and the polyclonal antibody and Goat anti mouse IgG were immobilized on nitrocellulose membrane to prepare an immunochromatographic strip device.Serum from 52 lung adenocarcinoma and 40 healthy controls(HC) were examined.Result can be obtained in 10～15 minutes after the serum samples for detection were added to the test strip.CEA,NSE,CYFRA2l-1 were measured by ECLIA for comparison.
其他文献
目的 检测限期手术的散发性结直肠癌(Colorectal cancer,CRC)患者肠道肿瘤组织粘膜和癌旁组织粘膜中莓实假单胞菌(Pseudomonas fragi,P.fragi)的含量.方法 选取上海市第十人民医院普外科2013年10月至2014年10月行限期CRC手术的散发性CRC患者22例,钳取肠道肿瘤组织粘膜和配对癌旁组织粘膜,液氮冻存;同时选取行结直肠疾病筛查的健康志愿者肠道粘膜活检标
目的 通过研究不同营养支持方法对术后切口愈合的影响,探索以口腔黏膜上皮脱落细胞凋亡率为评估手段的营养理论——"上限营养"方法,在手术后病人营养支持治疗中的可行性及有效性.方法选取53名胃癌患者,将其分为"公式营养组"和"上限营养组",进行前瞻性临床随机对照研究.测定两组患者术前1天至术后8天的口腔黏膜上皮脱落细胞的凋亡率.一组患者术后给予以Harris-Benedict公式为基础的"公式营养"支持
Background The purpose of this prospective study was to determine risk factors for readmission within 30 days of discharge after gastrectomy for patients with gastric cancer.Methods We conducted a pro
目的 观察酒精刺激对小鼠乳腺癌移植瘤生长与转移的影响,并探讨其作用机制.方法 利用SPF级C57BL/6雌性小鼠建立模拟人类饮酒的动物模型,并随机分为对照组和酒精组.对照组给予常规灭菌饮用水,酒精组每晚8点到次日早8点给予含有酒精的饮用水,每早8点到晚8点换回常规灭菌饮用水,依次循环;预处理3天后,将E0771髓样乳腺癌细胞皮下接种到小鼠下腹部乳房脂肪垫处,每3天观察肿瘤生长情况,于肿瘤细胞接种第
目的 比较腹腔镜技术联合加速康复外科理念对结直肠癌根治手术患者的安全性与有效性.方法 计算机检索PubMed、Embase、Cochrane library和中国生物医学数据库中所有有关腹腔镜手术联合加速康复外科理念有结直肠癌患者中应用的随机对照试验.结果 有7个随机对照试共714名结直肠癌患者纳入研究:腹腔镜手术联合加速康复外科理念组(LFT组)中有349名患者,开腹手术联合加速康复外科理念组中
目的 研究国产短肽型肠内营养对胃切除术患者营养状态及肠道适应性的影响.方法 采用随机数表将67例接受择期胃切除患者分为研究组(n=33)和对照组(n=34),本研究为双盲、随机对照研究,两组能量供给根据Harris-Benedict公式测算基础能量消耗(BEE),再乘以校正系数1.1,氮量供给0.2g/(kg·d),研究组给予国产短肽型肠内营养聚合剂(力存),对照组给予整蛋白型肠内营养聚合剂(能全
Laboratory testing plays a pivotal role in disease diagnosis and patient care.Approximate 85 ％of physicians decisions for diagnosis and therapy are based on clinical laboratory test results.Laboratory
A Laboratory Information Management System (LIMS),sometimes referred to as a Laboratory Information System (LIS) or Laboratory Management System (LMS),is a software-based laboratory and information ma
Rapid and accurate identification of a pathogen is of prime importance for effective disease treatment.Surface enhanced laser desorption & ionization time of flight mass spectrometry (SELDI-TOF MS) co
Objective Circulating tumor cells (CTCs) in peripheral blood are considered as a valuable biomarker for tumor metastasis and may be the origin of incurable metastatic disease.A label-free and high-sen