【摘 要】
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Ethnopharmacological relevance:Hypericum perforatum (St.Johns wort) is an important antidepressant herb used in clinic and commonly prescribed for mild depression.Hyperoside is one of the major compon
【机 构】
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Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese M
【出 处】
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中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会
论文部分内容阅读
Ethnopharmacological relevance:Hypericum perforatum (St.Johns wort) is an important antidepressant herb used in clinic and commonly prescribed for mild depression.Hyperoside is one of the major components of Hypericum perforatum and is also detected in many plant species such as Abelmoschus manihot,Black Currant,Rosa agrestis,Apocynum venetum and Nelumbo nucifera.Aim of the study:As hyperoside showed CNS (central nervous system) protective activity (e.g.antidepressant-like effect),the possibility of hyperoside or its metabolites to reach CNS should be investigated.Moreover,the pharmacokinetics profile of hyperoside or its metabolites in rat brain should be studied for further elucidating the mechanism of hyperoside action on CNS.Material and Methods:A simple method for simultaneous determination of unbound hyperoside and its metabolite 3′-O-methyl-hyperoside in rat brain was developed by using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) and microdialysis technique.
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