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A certain H5N1 avian influenza virus has gained the ability to cause the classic central nervous system dysfunction in poultry and migratory birds.This study presents the proteomics analysis on the change of proteins to H5N1 avian influenza virus with neurovirulence infection in chicken brain tissue.By using 2-DE,coupled with MALDI-TOF MS/MS,we identified a set of differentially expressed cellular proteins,including 18 up-regulated proteins and 13 down-regulated proteins.