【摘 要】
：
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV),the causative agent of porcine reproductive and respiratory syndrome,is responsible for a serious disease in pigs that
【机 构】
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State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Ec
【出 处】
：
2013国际猪繁殖与呼吸综合征学术研讨会
论文部分内容阅读
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV),the causative agent of porcine reproductive and respiratory syndrome,is responsible for a serious disease in pigs that ultimately results in substantial economic losses in the porcine industry.PRRS viruses are recognized to possess a high degree of genetic and antigenic variability.The purpose of this study was to evaluate the virulence of a modified live HP-PRRS TJM-F92 vaccine by serial passages in pigs and to analyze the genetic changes ofTJM-F92 virus during replication in pigs.Animals were inoculated withTJM-F92 vaccine virus derived from HP-PRRS TJ strain.Five passages of in vivo replication were maintained for 112 days by pig-to-pig passage.All animals were observed for clinical sighs and rectal temperature.The complete genomes of isolates from five animal passages (P1 to P5) were sequenced and compared to the TJM-F92 vaccine virus and its parent strain TJ.All pigs (from P1 to P5) showed no clinical symptoms related to PRRS although the viremic level in pigs from P 1 to P5 increased.Sequence analysis showed the continuous 120 amino acid deletion in nsp2 in TJM-F92 vaccine virus existed persistently in vivo,however,29 nucleotide changes resulting in 21 amino acid changes distributed in nsplβ,nsp2,nsp3,nsp9,GP3,GP4,GP5,and N during the experiment.Results showed that TJM-F92 vaccine virus did not show any reversion to virulence.
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