In asparagus (Asparagus officinalis L.) producing regions in Japan, F.oxysporum f.sp.asparagi and F.proliferatum are some of the most important causes as biotic factors which lead to asparagus decline.In this study, the diversity of Fusarium population which cause Fusarium crown and root rot in two asparagus decline fields was estimated by PCR-SSCP (single-stranded conformational polymorphism) method.