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Chlamydophila psittaci, an obligate intracellular pathogen, has been associated with psittacosis.Also, C.psittaci is a zoonotic agent.CPSIT_0846, a putative transmembrane head (TMH) family protein, was detected in the inclusion membrane during C.psittaci infection with antibodies raised with CPSIT_0846 fusion proteins by immunofluorescence assay.In the time-course monitoring, RT-PCR and Western blot revealed that it could be present as early as 2 h post infection (hpi), while it was visualized in inclusion membrane at 18 hpi in immunofluorescence assay, and remained in inclusion membrane throughout the growth cycle.CPSIT_0846 had indicated no significant orthologues with Chlamydia trachomatis and Chlamydophila pneumoniae, and in our experiment, recombinated CPSIT_0846 protein could not react with the serum of C.trachomatis and C.pneumoniae but could strongly recognize with the serum of C.psittaci, which revealed CPSIT_0846 is C.psittaci specific, so it could be used to diagnose human chlamydia disease cause by C.psittaci.Moreover, CPSIT_0846 could induce the expression of the inflammatory cytokines interleukin-1 Beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-oα) in THP-1 cells, which might be associated with chlamydia-induced inflammatory pathologies.