In some angiosperms, the zygotic division gives rise to two daughter cells with distinct morphologies and developmental fates.The apical cell develops into an embryo, and the basal cell mainly divides to form a suspensor that later completely disappears.It is still unknown when these distinct cell fates are determined and whether the basal cell fate determination and suspensor formation depend on its interaction with the apical cell or/and the embryo proper.Using an in vitro microculture system along with a laser-controlled microdissection technique (LCM), here we demonstrate that a single basal cell can follow basic standard embryonic pattern and develop into a filamentous suspensor.Morphological, cytological, and gene expression analyses confirmed that the resulting suspensor is highly similar to its in vivo counterpart.