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Artemisia annua L.is a popular medical plant used against malaria.Transgenic A.annua plants have been developed to increase the artemisinin content using geneticengineering.To develop a reliable PCR method of detecting transgenic A.annua,oneArtemisia specific gene,GEL4,was selected and validated as suitable.QualitativePCR methods were assayed with 16 different A.annua ecotypes and four Artemisiaplants,and identical amplified products were obtained.