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Sphingomonads DC-6 and DC-2 degrade the chloroacetanilide herbicides alachlor,acetochlor,and butachlor via N-dealkylation.In this study,we report a three-component Rieske non-heme iron oxygenase (RHO) system catalyzing the N-dealkylation of these herbicides.The oxygenase component gene cndA is located in a transposable element that is highly conserved in the two strains.CndA shares 24 to 42% amino acid sequence identities with the oxygenase components of some RHOs that catalyze N- or O-demethylation.