Runx2转录活性上调剂的筛选及其作用机制的初步研究

来源 :第四届中国药理学会补益药药理专业委员会学术研讨会 | 被引量 : 0次 | 上传用户：cwwei1

【摘要】

：

目的:应用本实验室建立的以Runx2为潜在作用靶点的高通量筛选模型,筛选具有上调Runx2转录活性的化合物,并对其作用机制进行初步研究,为开发新型抗骨质疏松药物提供先导化合物.方法:通过检测模型细胞中荧光素酶表达活性的变化,对化合物库进行了筛选,并利用p-NPP方法检测阳性化合物对MC3T3-E1碱性磷酸酶活性影响.同时检测阳性化合物对RANKL激活的RAW264.7细胞NF-κB转录活性的影响,

【作者】

：

赵晓丽陈金晶陈琳峰司书毅王真

【机构】

：

中国医学科学院医药生物技术研究所北京天坛西里一号100050 美国伊利诺伊大学香槟分校,美国伊利

【出处】

：

第四届中国药理学会补益药药理专业委员会学术研讨会

【发表日期】

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2014年3期

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