【摘 要】
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Visfatin has been seen as a biomarker in several kinds of cancers.However,no evidence has been reported for the direct effect of visfatin on osteosarcoma cells metastasis.The aims of this study were t
【机 构】
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Guangdong Provincial Key Laboratory of Orthopedics and Traumatology/Orthopedic Research Institute,Th
【出 处】
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中华医学会第十八届骨科学术会议暨第十一届COA国际学术大会
论文部分内容阅读
Visfatin has been seen as a biomarker in several kinds of cancers.However,no evidence has been reported for the direct effect of visfatin on osteosarcoma cells metastasis.The aims of this study were to investigate the influence of visfatin on the migration and invasion of osteosarcoma cells and clarify the underlying mechanism.The expressions of epithelial-mesenchymal transition(EMT)markers,as well as the transcriptional factor Snail1,were firstly detected at both protein and mRNA levels after the stimulation of visfatin.And then the expression of NF-κB(p65)was detected with western blot.Meanwhile,siRNA of Snail1 and inhibitor of the NF-κB were used to investigate the effect of visfatin.At last,migration and invasion of the cells were detected respectively by scratch wound healing assay and transwell assay.Visfatin down-regulated E-cadherin and up-regulated N-cadherin in concentration-and time-dependent manners at both protein and mRNA levels.And the expression of Snail1 was also up-regulated.Meanwhile,visfatin also promoted the nuclear translocation of NF-κB pathway.Administration of siRNA of Snail1 and the inhibitor BAY11-7082 validated the roles of Snail1 and NF-κB on the expressions of EMT markers.Migration and invasion of U2OS osteosarcoma cells were both ptomoted with the application of visfatin.These results demonstrate that visfatin enhanced migration and invasion of osteosarcoma cells via NF-κB/Snail/EMT pathway.
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