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Objective: To investigate the influence of Lycium barbarum polysaccharides (LBP) on the growth of human prostate carcinoma DU-145 cells and its mechanism.Method: The MTT assay was used to examine the inhibition effect of LBP on DU-145 cell, TUNEL was used to examine the apoptosis of DU-145 cells and Comet assay was applied to determine the DNA damage of DU-145 cell.The immunohistochemical technique was used to detect Bcl-2 and Bax expression.Result: LBP could inhibit the proliferation of DU-145 cell, induce DU-145 cell apoptosis and DNA damage, compared with the control, there was a significant difference and the ratio of Bcl-2/Bax protein decreased significant.Conclusion: LBP could inhibit DU-145 cell line in vitro, the mechanism may be related to its influence on inducing apoptosis, damaging DNA chain, and adjusting Bcl-2/Bax expression