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Three-day-old Sprague-Dawley rats were used in this study to provide static cultures of whole brain slices.Brain slices were randomly divided into control group, model group, empty plasmid transfection group and microRNA-9 (miRNA-9) transfection group.Except for the control group, the other three groups were cultured in sugar-free medium in an atmosphere of 8% oxygen + 92% nitrogen for 60 minutes, and the empty plasmid transfection group and miRNA-9 transfection group were transfected with empty plasmid or miRNA-9 plasmids.