Lepidopteran insects(moths and butterflies)are the second largest order of animals which also are probably the most widely studied groups of invertebrates.The Lepidoptera include numerous agricultural pests that cause widespread economic damage to commercial crops and trees,and the model insect with economic importance,i.e.the domesticated silkworm,Bombyx mori.How to manipulate Lepidoptera genomes is becoming the critical issue since a large number of insect genomic information has been released.Advances in genetic manipulation,notably transposon-based genetic transformation,have been successfully established in B.mori and applied extensively in gene function analysis and the production of bioreactors.Furthermore,successful establishment of genomic editing technologies including ZFNs,TALENs and CRISPR/Cas system provides a potential basis for improvements of insect functional genomics.Transgenesis combining genome editing technologies will greatly facilitate lepidopteran functional genomics and future genetic control of Lepidopteran insects.Works in our lab are focusing on: 1)dissection of Lepidopteran sex determination pathway and sex-specific regulatory elements using transgenic approach; 2)applications of transgene-based Sterile Insect Technology(SIT)in Lepidopteran insects; 4)applications of genome editing technologies,especially CRISPR/Cas9 system,in Lepidopteran functional genomics.