The enthusiasm of students to accept and use e-learning system will largely affect the process of the construction of educational informatization in universities.The purpose of this study is to explore key factors affecting students adoption of e-learning system based on Unified Theory of Acceptance and use of Technology (UTAUT).This study empirically validates the proposed model based on 295 valid questionnaires collected in the system using structural equation modeling (SEM).The results show that there are significant positive relationships between performance expectancy,effort expectancy,social influence,facilitating conditions and behavior intention,efforts expectancy significantly affects performance expectancy,facilitating conditions and behavioral intention has no significant impact on use behavior.Based on the discussion of this research results,we propose suggestions for the effective use of e-learning systems in higher education.