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Ma-Zi-Ren-Wan (MZRW), also known as Hemp seed pills, a formula first recorded in a TCM classic "ShanHanLun", has been widely used for constipation for many years.However, the constituents absorbed into blood after oral administration of MZRW remain unknown.In this study, an ultra-performance liquid chromatography-electrospray ionization-quadruple-time-of flight mass spectrometry (UPLC-ESI-QTOF-MS) technology coupled with Agilent MassHunter Workstation software was applied for the identification of the constituents in rat plasma after gavage administration of MZRW herbal extract.