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BIBAC system has been developed not only for structural genomics research as a BAC library, but also for functional genomics studies through Agrobacterium-mediated transformation of large genomic DNA fragments into plants.We previously modified BIBAC and BAC vectors (Shi et al., 2011, Plant Methods 7:33) that can largely facilitate BAC/BIBAC library construction and exchange of intact large insert DNA fragment between the BIBAC vector and the BAC vector.