【摘 要】
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Summary: Osteosarcoma,characterized by hypoxia which triggers HIF-1a induction,necessitates angiogenesis.Previous studies revealed that HIF-1a gene can be silenced by RNAi.However,under the HIF-1α gen
【机 构】
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Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University of Science and T
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Summary: Osteosarcoma,characterized by hypoxia which triggers HIF-1a induction,necessitates angiogenesis.Previous studies revealed that HIF-1a gene can be silenced by RNAi.However,under the HIF-1α gene silencing condition angiogenesis in osteosarcoma remains still under discussion.In this study,we further examined the SaOS-2 cells transfected with pSilencer2.1-HIF and found that in vitro,VEGF immunohistochemistry and the relative expression of VEGF(by qPCR)in SaOS-2/siRNA group were obviously reduced,and in vivo,morphological observation illustrated that SaOS-2/siRNA group cell heteromorphism was not obvious and vascular systems were sparse,immunohistochemistry revealed that both VEGF and CD34 were significantly decreased in SaOS-2/siRNA group,and tumor microvascular density(MVD)in SaOS-2/siRNA group(25.3±2.2)were obviously less than that in SaOS-2 group(58.1±2.5)(P< 0.05).
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