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Background: In addition to being highly infectious, Hepatitis B virus (HBV) is a major cause of liver disease worldwide.In hospital settings, it is easy for the environment and quilts to be contaminated by HBV patient blood and body fluids.Therefore, HBV can be transmitted to other patients via contaminated environmental surfaces or quilts, resulting in an HBV nosocomial infection.Formaldehyde and ozone are commonly used disinfectants that may influence this infectious situation.Objective: To investigate the clinical effectiveness of formaldehyde and gaseous ozone for the terminal cleaning of hospital quilts contaminated with HBV.Methods: Thin cloth and thick cotton soaked with the serum from high HBV copy number patients were prepared and disinfected using formaldehyde fumigation and gaseous ozone at different times.The copy numbers of HBV DNA in the HBV-contaminated cloth and cotton samples were measured quantitatively with fluorescent quantitative polymerase chain reaction (PCR).