This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products.Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge.The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions.Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins.The detection limits (S/N =3) for aflatoxin G1, B1, G2 and B2 were 0.03, 0.07, 0.05 and 0.09μg · kg-1 , and the corresponding quantification limits (S/N =10) were 0.10, 0.25, 0.18 and 0.30μg · kg-1 , respectively.The recoveries of aflatoxins in samples were 90.1%-104.4% and RSDs were <4.4%.The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79μg · kg-1.