In insomniac individuals, memory complaint is frequent but not unequivocally corroborated by objective evaluation.Whether it is involved in dysfunction of neuroendocrine remains unknown.In this study, 21 patients with chronic insomnia disorder (CID) and 25 patients with insomnia comorbid with depression disorder (CDD)were enrolled from the clinical setting, and 20 good sleepers were serviced as controls.The memories,including spatial working and reference memories, object working and reference memories, and object recognition memory,were evaluated using Nine Box Maze Test, and the serum hormones were measured.Compared to the controls, the CID patients had more errors in the spatial working and object recognition memories, whereas the CDD patients had poorer performance in all assessed memories.The CID patients had higher levels of corticotrophin-releasing hormone, cortisol and total triiodothyronine and thyroxine, and lower levels of thyrotropin-releasing hormone,gonadotropin-releasing hormone and adrenocorticotropic hormone than the CDD patients.However, the levels of all hormones were higher in the CID and CDD patients compared to the controls.For the associations between memories and hormones, after controlling confounding factors (age, sex, education, sleep and mood), only the CID patients, but not the CDD patients, showed few correlations, i.e., cortisol level correlated with the errors positively in the object working memory and negatively in the object recognition memory.Our results suggested that the CID patientshad selectively impaired memory, andincreased cortisol might direct.