【摘 要】
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Transplantation of olfactory ensheathing cells (OECs) becomes one of the promising strategies in restoring lost functions of injured central nervous system.It was revealed that OECs transplanted into
【机 构】
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Institute of Neurosciences, The Fourth Military Medical University, Xi'an 710032, China
【出 处】
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中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Transplantation of olfactory ensheathing cells (OECs) becomes one of the promising strategies in restoring lost functions of injured central nervous system.It was revealed that OECs transplanted into partially injured spinal cord have a distant in vivo neuroprotective effect on descending cortical and brain stem neurons.However, this study gave no answers to the question whether OECs can protect the central sensitive neurons with a closer axonal injury because different neurons respond variously to similar axonal injury and the distance between the neuronal soma and axonal injury site has a definite effect on the severity of neuronal response and apoptosis.In the present study, we investigated the effect of transplanted OECs on axotomized retinal ganglion cells (RGCs) after intra-orbital optic nerve (ON) transection in adult rats and possible in vitro mechanisms.Green fluorescent protein-OECs were injected into the ocular stumps of transected ON and a significantly higher number of surviving RGCs was found together with a consistent remarked increase in the mRNA and protein levels of BDNF within the ON stump and retina in OEC-treated group at 7, but not 2 and 14, day time point when compared to the control group.Furthermore, the effects of BDNF neutralization on the neuroprotection of adult OEC-conditioned medium (OEC-CM) on scratch-insulted RGCs were examined in vitro.The results showed that OEC-CM protected cultured RGCs from scratch insult, and neutralization of BDNF by BDNF neutralizing antibody attenuated such neuroprotection of the medium.Our findings suggest that OEC transplantation induces the expression of BDNF in the ocular ON stump and retina and delay the death of axotomized RGCs at a certain survival period.Neurotrophic factors including BDNF secreted by OECs can protect injured OECs in vitro and BDNF plays a major role in such a protection of OECs.
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