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Baculoviruses contain a large circular DNA genome ranging from 80-180 kb and have been widely used as bio-control agents and protein expression vectors.To date,recombinant baculoviruses have been constructed based on homologous recombination in either insect cells or in E coli.Here we report the first synthesis of AcMNPV,the prototype baculovirus,via transformation-associated recombination (TAR) in Saccharomyces cerevisiae.