Indoleamine 2,3-dioxygenase 1(IDO1)and tryptophan 2,3-dioxygenase(TDO)are constitutively overexpressed in many types of cancer cells and exert important immunosuppressive functions[1].
There is a great deal of interest in the development of cell-based system in diagnosis and treatment,and advances of it have provided a promising strategy for future medicine.
Super-resolution optical fluctuation imaging(SOFI)produces fast,background-free,super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters.