【摘 要】
：
目的 观察当归提取物AS-A、AS-B、AS-C、AS-D、AS-E、AS-F、AS-G和AS-B-1对PC12细胞生长分化的影响和对PC12细胞缺氧损伤的保护作用.方法 实验分为空白组、NGF组和给药组.NGF组作为阳性对照,终浓度为20 ng/mL,给药各组在缺氧前24 h分别按25 μg/mL、50 μg/mL和100 μg/mL浓度加入当归不同提取物.将正常培养8天的细胞,移置恒温(37℃
【机 构】
：
军事医学科学院基础医学研究所,北京100850;首都医科大学神经生物系,北京100069 军事医学
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
目的 观察当归提取物AS-A、AS-B、AS-C、AS-D、AS-E、AS-F、AS-G和AS-B-1对PC12细胞生长分化的影响和对PC12细胞缺氧损伤的保护作用.方法 实验分为空白组、NGF组和给药组.NGF组作为阳性对照,终浓度为20 ng/mL,给药各组在缺氧前24 h分别按25 μg/mL、50 μg/mL和100 μg/mL浓度加入当归不同提取物.将正常培养8天的细胞,移置恒温(37℃)密闭缺氧容器内,连续充以含90％N2和10％ CO2的无氧气体,在缺氧条件下继续培养12h,然后取出.取出的细胞均在倒置相差显微镜(400×)下每皿按Z字顺序随机观察并计数10个相邻视野的活细胞和死细胞数,计算PC12细胞存活百分率.结果 1.常氧情况下,NGF能促进PC12细胞的分化,当归不同提取物对PC12细胞的分化作用不明显；而提取物中,AS-A(100 μg/mL)、AS-C(100μg/mL)、AS-E(100 μg/mL)、AS-F(25 μg/mL、50 μg/mL、100 μg/mL) AS-G(100 μg/mL)和AS-B-1(100 μg/mL)对PC12细胞增殖有抑制作用.
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