【摘 要】
：
Alkyl, as a bioorthogonal chemical reporter, can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans.In addition to the clickable chemical reactivity
【机 构】
：
Department of Biophysics,School of Life Sciences,PKU,Beijing,China;Department of Chemical Biology,Co
【出 处】
：
8th IUPAP International Conference on Biological Physics(第八届
论文部分内容阅读
Alkyl, as a bioorthogonal chemical reporter, can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans.In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within the Raman-silent region, approximately 1800 to 2800 cm-1, of a cell.Since all natural cellular molecules do not produce Raman signals in this silent region, alkyl is Raman spectroscopically bioorthogonal,enabling specific and background-free detection.In this regards, we coupled this spectroscopic signature with Raman microscopy yields a new imaging modality beyond fluorescence and label-free microscopies.
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