It is believed that food allergens are proteins resistant to digestion.Digestibility tests have been accepted as an appropriate method for evaluating the allergenicity of newly introduced proteins.In this study we investigated the usefulness of this method for detecting the digestive stability of a major crustacean allergen (tropomyosin) and nonallergenic proteins scle of mud crab (Scylla serrata) in a standard simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestion assay system.Proteinases including pepsin,trypsin and chymotrypsin from porcine were used to simulate digestive proteinases from human.