【摘 要】
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CRISPR/Cas9 has been applied in human cells and many organisms for targeting a specific genome locus.Meanwhile, to efficiently enrich genetically modified c
【机 构】
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NorthwestA&FUniversity,Yangling712100,Shaan'xi
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CRISPR/Cas9 has been applied in human cells and many organisms for targeting a specific genome locus.Meanwhile, to efficiently enrich genetically modified cells, several surrogate systems have been developed.However, very limited information exists on the application of CRISPR/Cas9 in chickens.In this study, we used the CRISPR/Cas9 system to induce mutations in the PPAR-γ, ATP5E, and OVA genes at a rate of 0.75%, 0.5% and 3.0%, respectively, via a T7E1 assay in chicken DF-1 cells.
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