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目的通过完整exon2/3序列的测定,解决常规HLA-DRB1基因分型中的高比例歧义结果。方法采用常规的测序方法检测320份样本的HLA-DRB1外显子2第一高变区以外的序列,测序反应额外设置了codon86;采用1次性扩增外显子2/3,测序反应针对外显子2设置组特异性引物(DRB1*04/07/09为一组,其
Objective To resolve the high-level ambiguity results in the conventional HLA-DRB1 genotyping by determining the complete exon2 / 3 sequence. Methods Sequences other than the first hypervariable region of HLA-DRB1 exon 2 in 320 samples were detected by a routine sequencing method, codon86 was additionally set up in the sequencing reaction, exon 2/3 was amplified by one step, Exon 2 sets group-specific primers (DRB1 * 04/07/09 as a group