猪戊型肝炎病毒吉林地区分离株的全基因序列的分析

来源 :第十一届全国病毒学学术研讨会暨第六届武汉现代病毒学国际研讨会 | 被引量 : 0次 | 上传用户:qaz_wsx_123
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  目的:对2013年采自吉林地区养殖场的毒株编号为Ch-S-1的Ⅳ型HEV的全基因序列进行分析.方法:提取病毒RNA,采用RT-PCR方法分段扩增HEV全长基因,两端采用RACE法扩增,经过序列测定和拼接,利用ClusterX1.81、MEGA3.0、Phylop win 3.0软件分析基因序列.
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目的:了解山东省2011年一例急性驰缓性麻痹病例标本中分离到三株病毒的(脊髓灰质炎疫苗高变异株和柯萨奇病毒A4和A8病毒各一株)的基因特征,并对该病例接触者标本进行病原学鉴定及分析。方法:采集病例及40份接触者粪便标本进行病毒分离,对脊灰病毒进行全基因组序列测定和分析,对非脊灰肠道病毒进行VP1区序列测定,并将分离率较高的柯萨奇病毒(Coxsackievirus,CV)B1和CV-B3与国内外其他