【摘 要】
：
OBctivte:To study the preventive and therapeutic effects of AromaticChinese herbs (ACH) on IFV-infected Madin-Darby canine kidney (MDCK) cellsand IFV-infected mice.Methods:The antiviral activity of no
【机 构】
：
广州中医药大学,热带医学研究所,广州,中国 香港浸会大学,中医药学院,香港,中国
【出 处】
：
第三届民族传统医学与现代医学国际学术大会暨第十三次全国中西医结合防治呼吸系统疾病学术研讨会
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OBctivte:To study the preventive and therapeutic effects of AromaticChinese herbs (ACH) on IFV-infected Madin-Darby canine kidney (MDCK) cellsand IFV-infected mice.
Methods:The antiviral activity of nontoxic-level ACH against IFVA/FM/1/47 was examined by assaying (neutralization assay) its inhibitionof the IFV-induced cytopathic effects. The mode of ACH action was examinedwith a time-of-addition assay of synchronized infections,followed byviral attachment and penetration assays.MTT assay was used to evaluatemedian inhibitory concentration (ECM) and therapeutic index (TI).Inaddition,lung virus titers,lung index,lung histology,IFV load in lung,and the effect of ACH on pathogen recognition receptors of tracheal mucosawere detected.The inhibition of viral replication was demonstrated byquantitative real-time PCR,pathology technique and cell culturetechnique.
Conclusions: Our results give scientific support to the use of preventivetreatment with ACH against influenza virus.ACH (intranasal and aerosolroute) has potential utility in preventing and controlling seasonalpandemics of influenza virus infections.
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