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An ionic exclusion-enrichment phenomenon has been found at the ends of a nano-channel when electric-driven fluid passes through a micro-/nano-hybrid channel [1-3].In our experiments,the hybrid channels are fabricated with two poly-dimethysiloxane (PDMS) monoliths microchannels (100 m 20 m 9mm) and a nanoporous polycarbonate nuclear track-etched (PCTE) membrane (with 50nm pores).The flows are driven under different electrical potential and the test liquids with different PH values are used.The ion depletion in the source channel is observed by the MicroPIV system.In addition,the numerical simulations about ionic exclusion-enrichment in the hybrid channel are carried out.