【摘 要】
：
A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of eight constitu
【机 构】
：
Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese M
【出 处】
：
中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会
论文部分内容阅读
A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of eight constituents,uralsaponin C,uralsaponin F,22β-acetoxyl-glycyrrhizin,24-hydroxy-licorice-saponin E2,lieorice-saponin G2,licorice-saponin E2,glycyrrhizin,and licorice-saponin J2,from total saponin of licorice (TSL) in dog plasma.Ardisiacrispin A was used as the internal standard (IS).The separation was performed on Thermo Syncronic C18 column (100 mm×2.1 mm,1.7 μm) at a flow rate of 0.4 mL min-1,and acetonitrile/methanol (3∶1,v/v)-0.1% formic acid was used as mobile phase.The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM)via electrospray ionization (ESI) source with negative ionization mode.All calibration curves had good linearity (r > 0.991) over the concentration range from 2.03-405 ng mL-1 to 2.63-2625 ng mL-1 for all components.The intra-and inter-day precision was within 15% and the accuracy ranged from-14.08 % to 13.8%.The method was successfully applied to pharmacokiuetic study of eight triterpenoid saponins in dog plasma after oral administration of TSL.
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