Retinal diseases are the primary causes for visual impairment.One of the greatest challenges for retinal diseases therapy is low efficiency of topical administration.The purpose of this study is to prepare folate-conjugated silk fibroin nanoparticle to improve the bioavailability for retinal disease therapy.Folate receptor can be used as a moiety for enzyme-mediated cellular uptake because of its wide expression on the mammalian retinal pigmented epithelium and retina.