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A novel surface enhanced Raman scattering (SERS) substrate has been produced by combining Ag nanoparticles (AgNPs) and carbon nanocoils (CNCs).Three different methods have been developed for loading AgNPs on CNCs, which include (1) direct deposition of AgNPs on CNCs by radio-frequency magnetron sputtering (RFMS) to form Ag-CNC hybrid substrate, (2) deposition of a TiO2 film on CNCs by RFMS, on which photoinduced growth of AgNPs in an AgNO3 aqueous solution to form Ag-TiO2-CNC hybrid substrate (called A-substrate), and (3) deposition ofa TiO2 film on CNCs by spin-coating and then photoinduced growth of AgNPs in an AgNO3 aqueous solution to form Ag-TiO2-CNC hybrid substrate (called B-substrate).