【摘 要】
：
Strain XMU 706T,isolated from the rhizosphere soil of a herbaceous plant,Mirabilis jalapa L,collected from Xiamen City,China,was determined by a polyphasic approach to clarify its taxonomic position.S
【机 构】
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State-Province Joint Engineering Laboratory of Targeted Drugs from Natural Products,Xiamen Universit
【出 处】
：
第七届全国微生物资源学术暨国际微生物系统与分类学研讨会
论文部分内容阅读
Strain XMU 706T,isolated from the rhizosphere soil of a herbaceous plant,Mirabilis jalapa L,collected from Xiamen City,China,was determined by a polyphasic approach to clarify its taxonomic position.Strain XMU 706T shared the highest 16S rRNA genesequence similarity to Kribbella antibiotica YIM 31530T(97.2%),and formed a distinct branch in the subclade of the genus Kribbella in the 16S rRNA genephylogenetic tree.The genetic distances of gyrase subunit Bgene(gyrB)sequence between strain XMU 706r and other species of the genus Kribbella ranged from 0.045 to 0.116,greater than the threshold value 0.014 for species delineation of this genus.DNA-DNA hybridization experiments gave a DNA-DNA relatedness value of 34.82±6.31%between XMU 706T and K.antibiotica YIM 31530T.The chemotaxonomic properties further supported the assignment of strain XMU 706T to the genus Kribbella: LL-diaminopimelic acid was the diagnostic amino acid in the cell wall peptidoglycan,cell hydrolysates contained ribose and glucose.The major menaquinone was MK-9(H4).The polar lipids comprised diphosphatidylglycerol,phosphatidylglycerol,phosphatidylcholine and other unidentified phospholipids and lipids.The major fatty acids of the strain were anteiso-C15:0 and iso-C15:0,and the G+C content of genomic DNA was 67.3 mol%.Based on the results of phylogenetic analysis,phenotypic and genotypic characterization,strain XMU 706r represents a novel species in the genus Kribbella,for which the name Kribbella mirabilis sp.nov.is proposed.The type strain is XMU 706T(=KCTC 29676T=MCCC 1K00429T).
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