【摘 要】
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Background To investigate the effect of PDGFs/PDGFRs signaling pathway on the myocardial fibrosis in DOCA/salt hypertensive rats and its influence on cardiac fibroblasts and myofibroblasts.Methods Six
【机 构】
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Affiliated Anhui Provincial Hospital of Anhui Medical University,Hefei,Anhui,China
【出 处】
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第十二届中国介入心脏病学大会(CIT2014)
论文部分内容阅读
Background To investigate the effect of PDGFs/PDGFRs signaling pathway on the myocardial fibrosis in DOCA/salt hypertensive rats and its influence on cardiac fibroblasts and myofibroblasts.Methods Sixty male uninephrectomized SD rats were treated with 1％NaCL and 0.2％KCL in the drinking water for 4 weeks and randomly divided into three groups: vehicle control rats (CON group);DOCA treated group (DOCA group);DOCA and imatinib treated group (DOCA+IMA group).Systolic blood pressure (SBP) was measured weekly using the tail-cuff method;The extent of myocardial interstitial fibrosis and the ratio of PVCA/VA were analyzed by Sirius red staining;The rnRNA expressions of FSP-1,α-SMA,procollagen Ⅰ,procollagenⅢ,PDGFRα and PDGFRβ in the myocardiums was measured by RT-PCR;The protein levels of PDGFRα,PDGFRβ,p-PDGFRβ,vimentin and α-SMA were analyzed by immunohistochemical staining;The cell types of PDGFRα and PDGFRβ localizations were examined by immunofluorescence.
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