By taking Streptomyces griseorubens JSD-1 as the research object,effects of different kinds of surfactants on the production of cellulase from JSD-1 were investigated.Results show that the activity of cellulase has been significant increased,filter paper activity (FPA) and total protein were also higher when Tween-20 was added.That is because Tween-20 can promote the cell growth effectively by increasing the membrane permeability of the S.griseorubens JSD-1 and the secretion of extracellular enzymes,changing the fermented liquid surface tension and improving the oxygen transfer rate of the fermentation system.The added concentration of Tween-20,culture temperature and pH were optimized by using response surface analysis (RSA),and 1.45%,31.3℃,and 7.4 were determined,respectively.Under the optimum conditions,the cellulase activity reaches the highest value of 37.56 U/mL.